Characterization of guanosine diphospho-D-mannose dehydrogenase from Pseudomonas aeruginosa. Structural analysis by limited proteolysis.
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چکیده
منابع مشابه
In silico analysis of a therapeutic target in Leishmania infantum: the guanosine-diphospho-D-mannose pyrophosphorylase
Leishmaniases are tropical and sub-tropical diseases for which classical drugs (i.e. antimonials) exhibit toxicity and drug resistance. Such a situation requires to find new chemical series with antileishmanial activity. This work consists in analyzing the structure of a validated target in Leishmania: the GDP-mannose pyrophosphorylase (GDP-MP), an enzyme involved in glycosylation and essential...
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Strain GS, a gram-negative motile bacterium isolated from soil, synthesizes two polysaccharides. The synthesis of a uranic acid-containing polysaccharide is derepressed by high concentrations of Na2SOd, while the synthesis of the polysaccharide containing equal quantities of n-rhamnose and n-talomethylose is repressed by NazS01 (1, 2). L-F’ucose and L-rhamnose are the methylpentoses commonly fo...
متن کاملPurification and Characterization of Phosphomannose Isomerase - Guanosine Diphospho - D - mannose Pyrophosphorylase - A BIFUNCTIONAL ENZYME IN THE ALGINATE BIOSYNTHETIC PATHWAY OF PSEUDOMONAS
We report here the purification and characterization of phosphomannose isomerase-guanosine 5”diphospho-D-mannose pyrophosphorylase, a bifunctional enzyme (PMI-GMP) which catalyzes both the phosphomannose isomerase (PMI) and guanosine 5’-diphosphoD-mannOSe pyrophosphorylase (GMP) reactions of the Pseudomonas aeruginosa alginate biosynthetic pathway. The PMI and GMP activities co-eluted in the ...
متن کاملBiosynthesis of Guanosine Diphosphate D-rhamnose and Guanosine Diphosphate D-talomethylose from Guanosine Diphosphate Alpha-d-mannose.
Strain GS, a gram-negative motile bacterium isolated from soil, synthesizes two polysaccharides. The synthesis of a uranic acid-containing polysaccharide is derepressed by high concentrations of Na2SOd, while the synthesis of the polysaccharide containing equal quantities of n-rhamnose and n-talomethylose is repressed by NazS01 (1, 2). L-F’ucose and L-rhamnose are the methylpentoses commonly fo...
متن کاملCinnamide Derivatives of d‐Mannose as Inhibitors of the Bacterial Virulence Factor LecB from Pseudomonas aeruginosa †
Pseudomonas aeruginosa is an opportunistic Gram-negative pathogen with high antibiotic resistance. Its lectin LecB was identified as a virulence factor and is relevant in bacterial adhesion and biofilm formation. Inhibition of LecB with carbohydrate-based ligands results in a decrease in toxicity and biofilm formation. We recently discovered two classes of potent drug-like glycomimetic inhibito...
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ژورنال
عنوان ژورنال: Journal of Biological Chemistry
سال: 1992
ISSN: 0021-9258
DOI: 10.1016/s0021-9258(18)48384-6